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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
04/03/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MENA, E.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
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Afiliación : |
EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCOS MONTESANO, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Soybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733 |
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DOI : |
10.3389/fpls.2019.01733 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020.
The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826). |
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Contenido : |
Abstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses. MenosAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xyl... Presentar Todo |
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Palabras claves : |
CELL WALL; DEFENSE GENES; DIAPORTHE CAULIVORA; DISEASE SYMPTOMS; INTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA); PATHOGEN COLONIZATION; SOYBEAN STEM CANKER; TRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a). |
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Thesagro : |
ENFERMEDADES DE LAS PLANTAS; PATÓGENOS; SOJA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16699/1/fpls-10-01733.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2019.01733/pdf
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Marc : |
LEADER 03822naa a2200313 a 4500
001 1060886
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2019.01733$2DOI
100 1 $aMENA, E.
245 $aSoybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020. The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826).
520 $aAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses.
650 $aENFERMEDADES DE LAS PLANTAS
650 $aPATÓGENOS
650 $aSOJA
653 $aCELL WALL
653 $aDEFENSE GENES
653 $aDIAPORTHE CAULIVORA
653 $aDISEASE SYMPTOMS
653 $aINTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA)
653 $aPATHOGEN COLONIZATION
653 $aSOYBEAN STEM CANKER
653 $aTRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a)
700 1 $aSTEWART, S.
700 1 $aMONTESANO, M.
700 1 $aPONCE DE LEÓN, I.
773 $tFrontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733
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INIA La Estanzuela (LE) |
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| Registros recuperados : 12 | |
| 4. |  | MENA, E.; REBOLEDO, G.; STEWART, S.; MONTESANO, M.; PONCE DE LÉON, I. Análisis del transcriptoma de dos cultivares de soja en respuesta a la infección con Diaporthe caulivora. 306. (resúmen). Área temática: Biología Celular y Molecular. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.141-142. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 6. | | MENA, E.; GARAYCOCHEA, S.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. Comparative genomics of plant pathogenic Diaporthe species and transcriptomics of Diaporthe caulivora during host infection reveal insights into pathogenic strategies of the genus. BMC Genomics, 2022, volume 23, no. 1, 175. OPEN ACCESS. doi: https://doi.org/10.1186/s12864-022-08413-y Article history: Received 21 October 2021; Accepted 23 February 2022; Published 03 March 2022.
This work was funded by "Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowship)" Uruguay, "Programa...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | MENA, E.; REBOLEDO, G.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. O1. Análisis del transcriptoma de dos cultivares de soja en respuesta a la infección con Diaporthe caulivora. [Presentación oral]. Presentaciones orales. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 15. Financiamiento: Proyecto CSIC: "Receptores involucrados en la defensa vegetal frente a patógenos: Un abordaje evolutivo funcional y biotecnológico". Proyecto Fondo Clemente Estable FCE_3_2022_1_172688: "Estudio de los mecanismos de...| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 8. | | STEWART, S.; CORALLO, B.; BENTOS, D.; PONCE DE LEÓN, I.; PAN, D. P10. Patogenicidad de Fusarium spp. en sorgo. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 37. Financiamiento: Proyecto CSIC I+D 2020 "Patogenicidad y toxicidad de especies de Fusarium y su efecto en el rendimiento del cultivo de sorgo y la inocuidad de los granos". -- Autor correspondencia: e-mail: sstewart@inia.org.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 9. | | CORALLO, B.; MENA, E.; STEWART, S.; PONCE DE LEÓN, I.; PAN, D. P2. Expresión de genes de Fusarium graminearum implicados en la producción de toxinas. [Poster] Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 29. Financiamiento: proyecto CSIC I+D 2020. -- Autor correspondencia: e-mail: bcorallo@fcien.edu.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 10. | | MENA, E.; GARAYCOCHEA, S.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. Secuenciación del genoma y perfil transcriptómico de Diaporthe caulovora, agente causal del cancro del tallo en soja. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. p. 18.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Treinta y Tres. |
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| 11. | | GONZÁLEZ, A.C.; RUBIO, L.; MENA, E.; DELGADO-CERRONE, L.; ARRUABARRENA, A.; GONZÁLEZ-ARCOS, M.; PONCE DE LEÓN, I. P40. Identificación y caracterización de especies de Stemphylium causantes de la mancha gris de la hoja del tomate en Uruguay. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 66. Financiamiento: ANII beca de doctorado por proyecto FCE_1_2021_1_166555. -- Autor correspondencia: e-mail: anaclgonzalez92@gmail.com| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 12. | | CONDE, P.; GARCIA, C.; VILLAMIL, J.J.; IBÁÑEZ, F.; ZOPPOLO, R.; ARIAS-SIBILLOTTE, M.; PONCE DE LEÓN , I.; BORSANI , O.; GARCÍA INZA, G. The impact of irrigation on olive fruit yield and oil quality in a humid climate. Agronomy, 2022, vol.12, issue 2, e313. OPEN ACCESS. doi: https://doi.org/10.3390/agronomy12020313 Article history: Received 15 December 2021; Revised 4 January 2022; Accepted 11 January 2022; Published: 26 January 2022.
Academic Editor: José Casanova Gascón.
This article belongs to the Special Issue Plant Responses to Stress and...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 12 | |
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